The HPLC-UV analysis of caffeine and chlorogenic acid
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The method of analysis was developed for determination of caffeine and chlorogenic acid in coffee and cellular cultures.
HPLC-UV chromatogram of caffeine and chlorogenic acid standards.
Analysis of real coffee samples is more complex, there are also several isomeric compounds but all analytes were separated chromatographically and determined reliably and accurately.
Negative ESI LC-MS TIC (black) and PDA (blue) chromatograms of raw coffee bean extract (LC-MS Q-Exactive Plus).
Calibration curves for chlorogenic acid and caffeine obtained in HPLC-UV analysis.
UV spectra of caffeine and chlorogenic acid.
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