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The Q-Exactive high-resolution accurate-mass spectrometer is equipped with heated electrospray and atmospheric pressure chemical ionization ion sources. The mass spectrometer can be coupled with the UPLC-PDA for the analysis of many organic compounds. Additionally, it can be coupled with the Integrion ion chromatograph for highly sensitive and reliable analysis of polar organic acids and inorganic anions.
The instrument is used in metabolomic studies, analysis of environmental contaminants, and structure elucidations of unknowns.
Available software: Xcalibur, TraceFinder, Sieve.
Short description of the Q-Exactive operation principle
Ions generated in the API source are transferred through sets of lenses and flatapoles to a quadrupole mass filter (~10-5 Torr ) which can be used for isolation of wanted ions (SIM mode) or operated in RF-only mode for letting pass wanted ranges of m/z. From the quadrupole, ions are transferred and accumulated into the C-trap where their energy is dampened using a buffer nitrogen gas. From the C-trap, the ions are injected into the Orbitrap analyzer (~10-10 Torr ) where mass spectra are acquired via image current detection.
For the MS/MS analysis ions can be transferred through the C-trap into the HCD cell, where fragmentation takes place as a result of higher energy collisions with the nitrogen gas. The fragment ions are transferred back and accumulated into the C-trap from where they are injected into the Orbitrap for detection.
Ion optics scheme of the Q-Exactive mass spectrometer
High-resolution MS analysis is a superior technique for confirmation of atomic composition.
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