HPLC-UV/CAD analysis of ascorbyl tetraisopalmitate in cosmetic preparations

Vitamin C (L-ascorbic acid) is an effective antioxidant; it brightens the skin tone and reduces signs of aging.  Ascorbyl tetraisopalmitate is an oil-soluble, lipophilic derivative of Vitamin C that can be used in higher concentrations without problems. It is one of the most stable derivatives of Vitamin C but, being a derivative, its potency will not be directly comparable to pure L-ascorbic acid. 

Instrumental. The Ultimate 3000 HPLC system consists of a quaternary pump, autosampler, column compartment, photo-diode array and charged aerosol (CAD) detectors (Thermo Scientific). The compounds were separated on the Acclaim C30 column (2.1×150 mm, 3 µm,  Dionex) using a linear gradient of water/(acetonitrile/IPA 30:70).

Ascorbyl tetraisopalmitate was extracted from the cosmetic preparations with isopropanol.