HPLC-PDA/CAD (Thermo Scientific)
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The Ultimate 3000 HPLC system consists of a quaternary pump (620 bar), autosampler, column compartment, photodiode array (PDA), and charged aerosol (CAD) detectors (Thermo Scientific). The HPLC can be operated also in semi-preparative mode employing higher flow rates and wide-bore columns collecting eluted peaks using a fraction collector.
The PDA detector is used for many conventional HPLC analyses of compounds having appropriate chromophores.
The CAD can be used for the detection of any compound less volatile than the mobile phase solvents.
Available software, - Chromeleon.
Alkanes are usually analyzed using GC-FID or GC-MS techniques due to the good resolving capability of GC columns and the high sensitivity of FID or MS detectors. These non-polar compounds do not have chromophores or reactive chemical groups and cannot be detected by the photometric, electrochemical, pulsed-amperometric detectors, or ionized using ESI or APCI ionization. However, any compound significantly less volatile than a mobile phase can be detected using the charged aerosol detector.
HPLC-CAD chromatograms of alkanes mixtures (C44 RT=17.3 min; C40 RT=16.02 min; C38 RT=15.34 min; C36 RT=14.6 min; C34 RT=13.8 min; C32 RT=12.8 min ...)
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