LC-MS/MS analysis of plant hormones and growth regulators

We routinely analyze several common plant hormones in plants. Analysis of other growth regulators, present at significantly lower amounts, is not routine but can be discussed as a separate project.

Instrumental: QTRAP 6500+ mass spectrometer (Sciex) coupled with Nexera binary UPLC (Shimadzu). The mass spectrometer was operated in positive or negative ESI scheduled MRM mode. Chromatographic separations were carried out using the Acclaim C18 column (2.1×150 mm, 2.2 µm particle size, Dionex) using a linear gradient of ACN/water (with 0.1% acetic acid). The instrument was controlled and data were analyzed using Analyst and MultiQuant software respectively.