LC-MS/MS QTRAP 6500+ (Sciex)

Sciex QTRAP 6500+ mass spectrometer is coupled with Shimadzu Nexera X2 binary UPLC. The LC-MS/MS system is used for targeted quantitative analysis of environmental contaminants and in metabolomic studies.

The QTRAP 6500+ mass spectrometer proved itself as an exceptionally robust, sensitive, and reliable instrument for targeted quantitative analysis.

Available software: Analyst, MultiQuant.

Operation principles of the QTRAP

The first quadrupole in this series is the IonDrive QJet ion guide, located between the orifice plate and the Q0 region. The IonDrive QJet ion guide does not filter ions but focuses them before they enter the Q0 region. The IonDrive QJet ion guide helps to focus ions into the Q0 region. In the Q0 region, the ions are again focused before they enter the Q1 quadrupole.

The Q1 quadrupole is a filtering quadrupole that sorts the ions before they enter the Q2 collision cell. In the Q2 collision cell, the internal energy of an ion is increased through collisions with gas molecules until the molecular bonds break, creating product ions. After passing through the Q2 collision cell, the ions enter the Q3 quadrupole for additional filtering. Then they enter the detector. In the detector, the ions create a current that is converted into a voltage pulse i.e. the signal.

The Q3 quadrupole can be operated either as a quadrupole mass filter or as a linear ion trap. Thus, the QTRAP can also be used for MS3 analysis or other enhanced operations.

The LC-MS/MS QTRAP 6500+ applications

• Pesticides in water and sediments (positive mode)

• Pharmaceutical compounds in water and sediments (positive mode)

• Other environmental contaminants in water and sediments (negative mode)

• Analysis of pyrethroid insecticides in sediments

• Endo and phytocannabinoids in body fluids and tissues

• Analysis of free amino acids

• Analysis of plant hormones

• Steroids in biological samples